Effects of Shenling Baizhu powder on intestinal microflora metabolites and liver mitochondrial energy metabolism in nonalcoholic fatty liver mice

Background & purpose Non-alcoholic fatty liver disease (NAFLD) is characterised by the excessive accumulation of triglycerides in liver. Shenling Baizhu powder (SLBZP) formulated from various natural medicinal plants that protect and are used to treat intestinal diseases. SLBZP improves symptoms NAFLD. However, its mechanism action remains unclear. Herein, we investigated ameliorative effect on model mice with high-fat-diet (HFD)-induced Additionally, evaluated impact flora metabolites mitochondrial energy metabolism Methods We HFD establish a mouse Different drug interventions were administered. measured serum biochemical indices. Liver sections visualised hematoxylin–eosin oil red O staining. 16S rDNA amplicon sequencing technology was analyse diversity abundance flora. Short-chain acids (SCFAs) contents detected using GC-MS. tissue sampled detect membrane functional Western blotting determine levels pathway-related proteins, namely, uncoupling protein 2 (UCP2), adenosine monophosphate-activated kinase (AMPK) inhibitory factor 1 (IF1) F1Fo ATP synthesis/hydrolase, Results The spleen-invigorating classic recipe reduced lipid deposition HFD-induced SCFAs produced regulated UCP2/AMPK/IF1 signalling pathway involved improve permeability, respiratory state oxidative phosphorylation efficiency Finally, increased level. Conclusion Our results suggest therapeutic NAFLD related regulation hepatic possibly associated pathway.